Toluidine Blue Dye shows amazing foreign details in blood with weird implications.

Also a chance to get a 5MP USB3.0 microscope camera, see bottom of post for details!

While I am unable to post more today i will share an insight into what I have been working on recently. A dye i have been exploring the properties of called Toluidine Blue which has interesting very properties.

The Dye is known to be an indicator of many biological properties and some inorganic structures. It can detect or indicate the presence of DNA, RNA, Myelin which usually is a component of neurons and myelin sheath which covers the outside of nerve type material, it also can show amyloids when used in combination with polarized light microscopy, it can turn other colours for sulphates, and it can do more. The colours that are indicated by the dye's can be fairly accurate depending on the type of structures you are staining. With the material we are staining being rather exotic it is hard to say that the dye is deffinately showing us what the colours indicate, but it does infer strong possibility or likelihood that some of these structures are staining a relevent color.

One of the things which was most interesting is some of the dot like structures forming inside some blood cells. These dots dyed luminous yellow, usually an indication of myelin which oddly is neuron like material. But what's even more strange is the fibers that later formed and contained not only a yellow tint but also brightly coloured yellow dots inside which seemed much like the ones found forming in the RBC's. Now what is certain is that other blood cells contained dot structures which did not stain at all with this Dye. Those dots are obviously constructed of different materials. Many of the dots found loose in the plasma also did not stain with Toluidine Blue and previously other dyes so far have not adhered either which is pointing to those dots as certainly being along the lines to nanospheres of some kind and not biological or organic in nature. There were larger unshapely dot like structures that are possibly part organic which reacted to the dye as luminous a green. Clusters of varying nano particles or nanospheres seemed to show multiple colours when bonded in large masses, indicating material variences.

I will elaborate more with updated images when I do the next post. There seems to be the possible detection of RNA or DNA in some of the lipd structures which needs more attention. Cross staining with other dye's will help to confirm these preliminary indications. But interpreting these indicators correctly is proving to be trickier than standard procedures given that the material is unknown but also a mixture of unusual organic and inorganic nature. I have been sharing this work with qualified chemistry scientists and seeking there opinion on what these dyes may be inferring and how likely that accuracy may or may not be. Literature tends to refer to colour coding in samples of known conditions, on known and tested structures in their expected envirement.

So let's keep this post brief until I update with the other great images which look very suspect, and with images of newer samples too.

A PRIZE DRAW FUND RAISER IS IN HAND!

An Amscope/Omax 5mp USB3.0 high speed microscope camera which I had used a few times for only one week will be going for grabs. The camera retails for around $280. The quality is pretty good for the price and the images or video is fairly crisp. It comes with the supplied USB containing the software, a USB3.0 cable, a micrometer calibration slide, and the 0.5x c-mount reduction lense adapter to fit a standard eyepiece.

The story is that I thought I'd check out how good it was and decided quickly that my leica microscope of now nearly $14,000 deserved a much better image for what I was wanting to do and so I purchased a dearer one instead.

There is no warranty unfortunately but it comes boxed as new and I shall take images of what's for grabs and share the microscopy quality one would expect from the imaging camera.

I figured those who want to enter for a chance to win over a 1 week period can donate $20 upwards in order to get it. Any delivery or shipping over $50 will have to be covered by the lucky person. If only one person donates $20 it still goes to that person.

That person will receive an email to follow up on delivery details and any extra shipping charge above the $50 amount covered.

A name will be selected from KO-FI or PayPal via a fair system. Maybe I will get people to choose random numbers and letters in comments and take which ever of these details correlate to a persons donation by that criteria. We could do a draw post for this at a set time to make it even more fun!

If people are interested in this idea let me know below and I shall make it happen.

Thank you for supporting the work, and I wish everyone good health!

PLEASE consider helping to fund new equipment and research. I couldn't add the button for that via phone today so please see any other post for the KO-FI button at the bottom if you feel compelled . :D