Karl: I have no idea what kind of sample prep / handling is involved with these images. Is a cover slip used? Is there fluid extruding beyond cover slip thats exposed to drying? Has your mike been "on" for a while which warms it and the sample on the slide? What this looks like to me is water vapor condensing on the underside of the cover slip, and when large enough to touch the bottom surface, the drop gets sucked down. They "grow" and then suddenly "shrink", typical of condensation drops. I recommend using a large cover slip for every sample, which reduces drying from edge. Gently allow the cover slip to drop down onto sample using a needle or similar probe to minimize bubbles getting trapped. Also, keep them cool. Warming the slide in itself causes fluids to move / stream towards edges. This also causes clumping of RBCs. Uniform sample handling is all important here!
Oh boy... I don't off top my head Damon. My own "link" comes from microscopy over the last 60 years - lol. However, the right technique depends on what sort of samples you are working with. Blood can be very picky as its "live" and it wants to clot, especially outside the body. A lot of things can trigger blood clots: temp changes, vibration, unclean slides or coverslips, foreign stuff that might rupture an RBC, etc. etc. When laying coverslips down on blood, you never want to put the edge of the coverslip into blood, as you will rupture a few cells which sets off a chain reaction. Set the coverslip onto the clean microscope slide, and let the blood drop spread out slowly as you let the slip down gently. You can use a toothpick to let the coverslip down gently and that also avoids most bubbles.
Other samples: A sample of pond water to observe protozoans is pretty forgiving. Things like preparing plant thin-sections gets pretty involved with fixing and staining, plus embedding, and there are whole books and courses covering this topic. I also deal with thin sections of soil clods to observe mineral translocation in the soil profile - which is another whole subject in itself. If you are focused on observing live blood samples, I would focus on searching for standard techniques used in hematology labs. I'm sure you will find more info than you need. For determining "blood counts" you will need special equipment: a hemocytometer, which is a calibrated chamber of specific volume, so you can arrive at RBCs/ml of blood, etc. Hope this helps.
Maybe you should get involved. You have a broad range range of microscopy skills that no doctor alone has. All these different concepts used in other fields are ones i try to apply to this newer blood phenomena since it is unusual in nature as far as human biology goes. The finite skills you posses are essential to making clean, precise, and new observations. i am impressed. Do stay in contact if you wouldn't mind. cheers !
Thanks. My microscopy has been done using a provider but I have recently purchased one and will be applying myself once it arrives. Some of the reading I had done explained that poor handling can generate abnormalities in the blood.
For now i am too. This is more of a note of possibly suspect phenomena since other sittings have shown very strange behavior of other kinds going on in these bubble structures. At times a full morphological set of features seems to be occurring which warrants further investigation in my books..
Thank you. this is god advice. This was under a large cover slip on day 2. These same droplets we've also seen moving along like amoeba. Not to say that they are amoeba. Given the fact there are highly water absorbent gels apparent in the sample i am inclined to find this behavior suspicious. Contact with upper surface does not seem to be clearly apparent, although not scientific in assuming so either. I will have to upload other videos with time lapse behavior of these bubbles and one point so others can see the strange flags here.
My intent is to get involved much as I can help out. Time is of essence. Would like to correspond w/Ana but no luck in that dept. However, when I became "paid" member of her stack, I got a tiny allotment of "contact" with her. This is where the topic of birefringence suddenly came from. She is using dark field which is all reflected light, like birefringence. But, now I learn the micro dots might be florescent so can produce own light. I am convinced their movement is by Brownian motion. The jerky movement is typical of motion induced by molecular vibration. Cooling the sample should slow it down (temp dependent).
FYI: my Leitz scope is currently sent off for thorough cleaning and alignment. My father used it in his late years and some things need repair. I was offered a nice Leitz Pol scope for reasonable that I might pick up. I only have 3 scopes, so whats one more - lol. My optics are good but not new. It's all either Leitz or Zeiss, but I dont have the newer plan objectives, phase contrast, etc. I'm aware Ana has had trouble with her scope. Would like to see her trade up to a top of line Leica with all bells and whistles, but they run about 100k depending on whats included. Maybe we can garner funding to make it happen. Dont know if she has interest, but she might also want to get a good used SEM to be able to see the micro-dots. I have the same goal, but dont know how far I'll get using Pol scope and geological embedding and sectioning techniques, if I can get some of them concentrated for embedding. Those things are so small I dont know if I can get a few of them sliced open within the embedding epoxy. One more swipe even with super fine grit grinding compound will probably just grind out the whole thing - dont know. This is the wrong technique for something that small, but I'll try. This is why I suggest an SEM. I used one back in grad school, but the one I used is likely a museum piece now. Wouldn't mind being involved with that if it comes to it.
In terms of somehow making progress against mRNA, thats a tall order but I'll do what I can to contribute. I sense a large part of this battle is against inanimate materials - the micro dots and the rubber strand formation. I used to do blood electrification, but now dont dare as the strands grow faster under voltage. FYI, this process uses low voltage on skin over blood vessels to kill off parasites bacteria and viruses in blood. This very useful tool is now out the window. What next I dont know. Ana is on track using EDTA etc, but this is also rough on body as I understand. However, the plant world is full of enzymes we haven't tried yet. Some might be better than EDTA. I dont have a lab available here so cant really help outside of making suggestions. I'm experienced in wet soil chemistry, which is complex soup of redox reactions. I'm hoping this knowledge might be useful in deterring strand formation. I think strand formation involves electromagnetic charges that attract material in the growth process. We need to turn this off, or make use of it for filtration.
Look like anaesthetic videos by Dr. Nixon: gulping small bubbles, backward movement, pushhing forward and eventually exploding. The latter not being observed in yours.
Karl: I have no idea what kind of sample prep / handling is involved with these images. Is a cover slip used? Is there fluid extruding beyond cover slip thats exposed to drying? Has your mike been "on" for a while which warms it and the sample on the slide? What this looks like to me is water vapor condensing on the underside of the cover slip, and when large enough to touch the bottom surface, the drop gets sucked down. They "grow" and then suddenly "shrink", typical of condensation drops. I recommend using a large cover slip for every sample, which reduces drying from edge. Gently allow the cover slip to drop down onto sample using a needle or similar probe to minimize bubbles getting trapped. Also, keep them cool. Warming the slide in itself causes fluids to move / stream towards edges. This also causes clumping of RBCs. Uniform sample handling is all important here!
Hi mate
Would you have a link with a good how to for the best handling practices?
Oh boy... I don't off top my head Damon. My own "link" comes from microscopy over the last 60 years - lol. However, the right technique depends on what sort of samples you are working with. Blood can be very picky as its "live" and it wants to clot, especially outside the body. A lot of things can trigger blood clots: temp changes, vibration, unclean slides or coverslips, foreign stuff that might rupture an RBC, etc. etc. When laying coverslips down on blood, you never want to put the edge of the coverslip into blood, as you will rupture a few cells which sets off a chain reaction. Set the coverslip onto the clean microscope slide, and let the blood drop spread out slowly as you let the slip down gently. You can use a toothpick to let the coverslip down gently and that also avoids most bubbles.
Other samples: A sample of pond water to observe protozoans is pretty forgiving. Things like preparing plant thin-sections gets pretty involved with fixing and staining, plus embedding, and there are whole books and courses covering this topic. I also deal with thin sections of soil clods to observe mineral translocation in the soil profile - which is another whole subject in itself. If you are focused on observing live blood samples, I would focus on searching for standard techniques used in hematology labs. I'm sure you will find more info than you need. For determining "blood counts" you will need special equipment: a hemocytometer, which is a calibrated chamber of specific volume, so you can arrive at RBCs/ml of blood, etc. Hope this helps.
Maybe you should get involved. You have a broad range range of microscopy skills that no doctor alone has. All these different concepts used in other fields are ones i try to apply to this newer blood phenomena since it is unusual in nature as far as human biology goes. The finite skills you posses are essential to making clean, precise, and new observations. i am impressed. Do stay in contact if you wouldn't mind. cheers !
Thanks. My microscopy has been done using a provider but I have recently purchased one and will be applying myself once it arrives. Some of the reading I had done explained that poor handling can generate abnormalities in the blood.
Appreciate your response
I’m going with your explanation since the alternative is freaky!
For now i am too. This is more of a note of possibly suspect phenomena since other sittings have shown very strange behavior of other kinds going on in these bubble structures. At times a full morphological set of features seems to be occurring which warrants further investigation in my books..
Thank you. this is god advice. This was under a large cover slip on day 2. These same droplets we've also seen moving along like amoeba. Not to say that they are amoeba. Given the fact there are highly water absorbent gels apparent in the sample i am inclined to find this behavior suspicious. Contact with upper surface does not seem to be clearly apparent, although not scientific in assuming so either. I will have to upload other videos with time lapse behavior of these bubbles and one point so others can see the strange flags here.
My intent is to get involved much as I can help out. Time is of essence. Would like to correspond w/Ana but no luck in that dept. However, when I became "paid" member of her stack, I got a tiny allotment of "contact" with her. This is where the topic of birefringence suddenly came from. She is using dark field which is all reflected light, like birefringence. But, now I learn the micro dots might be florescent so can produce own light. I am convinced their movement is by Brownian motion. The jerky movement is typical of motion induced by molecular vibration. Cooling the sample should slow it down (temp dependent).
FYI: my Leitz scope is currently sent off for thorough cleaning and alignment. My father used it in his late years and some things need repair. I was offered a nice Leitz Pol scope for reasonable that I might pick up. I only have 3 scopes, so whats one more - lol. My optics are good but not new. It's all either Leitz or Zeiss, but I dont have the newer plan objectives, phase contrast, etc. I'm aware Ana has had trouble with her scope. Would like to see her trade up to a top of line Leica with all bells and whistles, but they run about 100k depending on whats included. Maybe we can garner funding to make it happen. Dont know if she has interest, but she might also want to get a good used SEM to be able to see the micro-dots. I have the same goal, but dont know how far I'll get using Pol scope and geological embedding and sectioning techniques, if I can get some of them concentrated for embedding. Those things are so small I dont know if I can get a few of them sliced open within the embedding epoxy. One more swipe even with super fine grit grinding compound will probably just grind out the whole thing - dont know. This is the wrong technique for something that small, but I'll try. This is why I suggest an SEM. I used one back in grad school, but the one I used is likely a museum piece now. Wouldn't mind being involved with that if it comes to it.
In terms of somehow making progress against mRNA, thats a tall order but I'll do what I can to contribute. I sense a large part of this battle is against inanimate materials - the micro dots and the rubber strand formation. I used to do blood electrification, but now dont dare as the strands grow faster under voltage. FYI, this process uses low voltage on skin over blood vessels to kill off parasites bacteria and viruses in blood. This very useful tool is now out the window. What next I dont know. Ana is on track using EDTA etc, but this is also rough on body as I understand. However, the plant world is full of enzymes we haven't tried yet. Some might be better than EDTA. I dont have a lab available here so cant really help outside of making suggestions. I'm experienced in wet soil chemistry, which is complex soup of redox reactions. I'm hoping this knowledge might be useful in deterring strand formation. I think strand formation involves electromagnetic charges that attract material in the growth process. We need to turn this off, or make use of it for filtration.
Its like an LCD display almost! Very interesting!
Look like anaesthetic videos by Dr. Nixon: gulping small bubbles, backward movement, pushhing forward and eventually exploding. The latter not being observed in yours.
Perhaps a chemical reaction that off gasses?